Sam successfully completed his Masters project with us, presenting a great poster on his study of peptide-lipid interactions by molecular dynamics simulations. Congratulations!
Small Heat Shock proteins such as HspB1 are molecular chaperones in charge of preventing harmful misfolding of proteins under stress conditions. Work led by the Benesch and Gehmlich groups shows, from muscle fibers down to single molecules, that phosphorylation of HspB1 alters its intramolecular dynamics, facilitating its binding to the mechanosensitive Filamin C. In good correspondence with NMR data, our calculations reveal that over over the course of microsecond-long simulations the N-terminus of HspB1 detaches from the rest of the protein.
Collier M.T., Alderson, T.R., de Villiers, C.P., Nicholls, D. , Gastall, H.Y., Allison, T.M., Degiacomi M.T., Jiang, H., Mlynek, G., Fürst, D.O., van der Ven, P.F.M., Djinovic-Carugo, K., Baldwin, A.J., Watkins, H., Gehmlich, K., Benesch, J.L.P. (2019), HspB1 phosphorylation regulates its intramolecular dynamics and mechanosensitive molecular chaperone interaction with filamin C. Science Advances, 5(5)
The image shows the structure of the long-lived small heat-shock proteins (present in the eye lens), and the effect of age-related isomerisation of key amino-acids, as detailed in our article.
[image composed with Gimp from protein VMD rendering redesigned by the artistic hand of Valentina Erastova]
In recent years, generative neural networks have been gaining popularity owing to their ability to produce believable fake data including photos, video and even news. The creativity of these neural networks lies on their capacity of generating something new based on collection of examples provided as input. We show that autoencoders, a kind of neural network, can be exploited to generate meaningful protein conformations.
Protein molecular function and malfunction in an organism is often linked to their interconversion between states caused by changes in environmental conditions or binding of ligands such as drugs or other proteins. In this context, we demonstrate two possible usages for an autoencoder: (1) predicting the transition path between two states sampled by MD simulations, when no sampling of the intermediates is available; (2) coupling the autoencoder with POWer, our protein docking algorithm, to help the prediction of proteins’ arrangement into a complex when the subunits undergo substantial conformational change.
Long-lived proteins may accumulate a range of modifications over time, including subtle alterations such as side-chain isomerization.
In collaboration with the
Julian group in UC Riverside and Benesch group in Oxford we study the effect of isomerisation of an aspartate residue in αB-crystallin, the most abundant chaperone proteins in the eye lens and within the longest-lived proteins in the body. Malfunction of these proteins is linked to a range of diseases, including cataract.
Our results illustrate how age-related isomerization of amino acid residues, which may seem to be only a minor structural perturbation, can disrupt native structural interactions with profound consequences for protein assembly and activity.
Lyon, Y.A., Collier, M.P., Riggs, D.L., Degiacomi, M.T., Benesch, J.L.P., Julian, R.R. (2019). Structural and functional consequences of age-related isomerization in α-crystallins, Journal of Biological Chemisty
Matteo attended the conference “celebration of native mass spectrometry” in Oxford, where he presented his protein integrative modelling methods leveraging on mass spectrometry data. An exciting venue, and a great opportunity meet old friends from the mass spec community!
Matteo then joined Venkat for a trip to London, where they visited Prof. Vittorio Bellotti at the Royal Free Hospital. Matteo delivered a presentation on means for computational modelling to inform biomedical research.
Lucas has won a prize for his flash talk “Density Based Protein Docking” at the
4th N8 Byophysical and Biochemical Symposium in Sheffield. Congratulations!!!
Lucas and Matteo attended the 4th N8 Byophysical and Biochemical Symposium in Sheffield. Matteo gave an invited talk about the group’s work on integrative modelling, and Lucas presented a poster and a flash talk about his development of an accurate scoring function for protein docking.
Our image was selected as a cover article for the 21st December edition of JBC!
The image represents different orientations of the tetrahedral assembly of a wheat small heat shock protein (sHSP). These proteins form dimers, that in turn assemble in more complex architectures.
In our work we show that sHSP dimers (a single one highlighted in yellow in the image) are in equilibrium with the oligomeric form and act as the initial encounter species to capture denaturing substrates.
[image produced with VMD with Tachyon ray tracing, and composed with Gimp]
Our work, proposing a plausible mechanism for the origin of prebiotic peptides, is featured in Nature Communications’ “Early Earth Collection”! This collection summarizes recent published work aimed at characterizing early earth conditions, and the crucial though still hardly understood transition from geo to biochemistry. See the editorial here!